Description
Coombs -Direct Antiglobulin Test
Sample Type: Whole Blood
Sample Required:
Blood sample.
Test Time:
Results typically available within a few days.
What is the test?
The Coombs direct antiglobulin test detects antibodies or complement proteins that are attached to the surface of red blood cells. It’s used to diagnose conditions related to autoimmune hemolytic anemia, hemolytic disease of the newborn, or other immune-mediated hemolytic disorders.
Test Procedure:
Blood is drawn from a vein, and the red blood cells are mixed with antibodies against human globulins. If the red blood cells have antibodies or complement proteins attached, they agglutinate (clump together), indicating a positive test.
When to take the test:
When there’s suspicion of autoimmune hemolytic anemia or hemolytic disease of the newborn.
Who should take the test:
Individuals with symptoms of anemia, jaundice, or a history of blood transfusions might undergo this test.
Precautions for exceptional cases (pregnancy, etc.):
Pregnant individuals might undergo this test if there’s a concern about hemolytic disease of the newborn.
Frequently Asked Questions (FAQs) for Coombs – Direct Antiglobulin Test (DAT):
Q1: What conditions can a positive DAT indicate?
A: It can indicate immune-mediated hemolytic anemias, transfusion reactions, or hemolytic disease of the newborn.
Q2: Can medications or infections affect the results of DAT?
A: Yes, certain medications or infections can cause a positive DAT, so it’s essential to consider the individual’s medical history.
Q3: How is DAT different from the indirect Coombs test?
A: DAT detects antibodies or complement proteins already attached to red blood cells, while the indirect Coombs test detects antibodies in the plasma that can attach to red blood cells.
Q4: Can a positive DAT mean a person has an autoimmune disorder?
A: Not necessarily. While it’s associated with autoimmune hemolytic anemia, a positive result requires further investigation to confirm the diagnosis.
Q5: Is the DAT a routine part of blood tests?
A: It’s not typically part of routine blood tests but is ordered when specific conditions are suspected.
*Please note that specific testing protocols and guidelines may vary based on the latest recommendations from health authorities and regulatory agencies. It is essential to consult healthcare professionals and follow local testing guidelines for accurate and appropriate testing.
