Description
Coombs test – Indirect Antiglobulin
Test
Sample Type: Serum
Sample Required:
Blood sample (typically collected from the recipient)
Test Time:
Results typically available within a few days.
What is the test?
The Coombs indirect antiglobulin test detects antibodies in the plasma that can attach to red blood cells. It’s used to identify antibodies in the blood that could cause destruction of red blood cells, aiding in the diagnosis of conditions like autoimmune hemolytic anemia or investigating compatibility for blood transfusions.
Test Procedure:
Blood is collected from the recipient, and the plasma is separated and mixed with known red blood cells. If the antibodies in the plasma react with the red blood cells, it indicates a positive test.
When to take the test:
When investigating the cause of hemolytic anemia or determining compatibility for blood transfusions.
Who should take the test:
Individuals with symptoms of hemolytic anemia or those needing blood transfusions might undergo this test.
Precautions for exceptional cases (pregnancy, etc.):
Pregnant individuals might undergo this test if there’s a concern about potential issues related to blood compatibility during pregnancy.
Frequently Asked Questions (FAQs) for Coombs Test – Indirect Antiglobulin Test (IAT):
Q1: What conditions can a positive IAT indicate?
A: It can indicate the presence of antibodies in the blood that might cause destruction of red blood cells, such as in autoimmune hemolytic anemia or transfusion reactions.
Q2: Is the IAT routinely done before blood transfusions?
A: Yes, it’s often performed to ensure compatibility between the recipient’s plasma and the donor’s red blood cells.
Q3: Can medications or infections affect the results of the IAT?
A: Yes, certain medications or infections can lead to positive IAT results, so it’s important to consider the individual’s medical history.
Q4: How does the IAT differ from the DAT?
A: The IAT detects antibodies in the plasma that could potentially react with red blood cells, whereas the DAT detects antibodies already attached to red blood cells.
Q5: Can a positive IAT affect pregnancy?
A: It might be significant if there’s a risk of hemolytic disease of the newborn due to blood group incompatibility between the mother and baby.
*Please note that specific testing protocols and guidelines may vary based on the latest recommendations from health authorities and regulatory agencies. It is essential to consult healthcare professionals and follow local testing guidelines for accurate and appropriate testing.
