Description
Blood, Human Leukocyte antigen B27 (HLA- B27) belongs to a group of cell surface glycoproteins which are expressed on most nucleated human cells and platelets. It is a major histocompatibility complex (MHC) class I molecule. The presence of HLA – B27 is strongly associated with ankylosing spondylitis and some inflammatory disorders like Reiter’s syndrome, inflammatory bowel syndrome, etc. The binding ability of specific monoclonal antibodies to the antigenic determinants expressed by leucocytes on the surface forms the basis of this test. HLA-B 27 test is generally advised in patients suffering from joint pain where the diagnosis of ankylosing spondylitis is suspected. A positive test result indicates higher chances of developing this disease. Avail of the service by booking the HLA-B27 test online at an affordable rate, at your doorstep., Non Fasting
Sample Required:
This test requires a blood sample. A blood sample will be drawn from a vein in your arm by a healthcare expert.
Test Time:
The HLA-B27 by PCR test typically takes several days to provide results, as it involves a complex laboratory analysis.
Test Normal Range:
The HLA-B27 test doesn’t have a conventional “normal range” like some other tests. Instead, the result is typically reported as positive or negative. A positive result indicates the presence of the HLA-B27 gene, while a negative result suggests its absence.
What is the Test?
The HLA-B27 by PCR test is a genetic test that detects the presence of the human leukocyte antigen B27 (HLA-B27) gene. This gene is associated with several autoimmune diseases, particularly ankylosing spondylitis and other spondyloarthropathies. The test helps in the diagnosis and management of these conditions.
Test Procedure:
A healthcare professional collects a blood sample from a vein in your arm. The blood sample is sent to a laboratory for genetic analysis. In the laboratory, PCR (polymerase chain reaction) is used to amplify and detect the HLA-B27 gene.
When to Take the Test:
The HLA-B27 by PCR test is usually taken when a healthcare provider suspects or needs to confirm a diagnosis of ankylosing spondylitis or other related conditions. It may also be used to assess the risk of developing these conditions in certain individuals.
Who Should Take This Test:
Individuals who are experiencing symptoms such as chronic back pain, joint pain, stiffness, or other symptoms suggestive of spondyloarthropathies may be recommended for this test. Additionally, individuals with a family history of these conditions might consider testing to assess their risk.
Precautions for Exceptional Cases:
There are no specific precautions related to pregnancy or other exceptional cases for this test. However, individuals should always inform their healthcare provider of any medical conditions, medications, or allergies before undergoing any testing.
FAQs (Frequently Asked Questions):
Q1: What are the conditions associated with HLA-B27 positivity?
A: HLA-B27 positivity is strongly associated with ankylosing spondylitis, reactive arthritis, and other spondyloarthropathies.
Q2: Can HLA-B27 testing predict the severity of a condition?
A: No, HLA-B27 testing indicates the presence or absence of the gene but doesn’t predict disease severity. It’s just one factor considered in diagnosis.
Q3: Is HLA-B27 testing required for everyone with back pain or joint symptoms?
A: No, HLA-B27 testing is typically ordered by a healthcare provider when there are clinical indications or a strong suspicion of spondyloarthropathies. It’s not a routine screening test.
Q4: Can I have HLA-B27 without having any symptoms or diseases?
A: Yes, some individuals can be HLA-B27 positive without having symptoms or diseases associated with it. The gene is a risk factor, but not everyone with the gene develops these conditions.
Q5: Can HLA-B27 testing diagnose ankylosing spondylitis by itself?
A: No, a diagnosis of ankylosing spondylitis or related conditions is typically based on a combination of clinical symptoms, physical examination, medical history, and sometimes imaging studies. HLA-B27 testing is part of the diagnostic process but not definitive on its own.
